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The prefrontal cortex and whole hypothalamus were immediately dissected out and flash frozen on dry ice

n = 8 for vehicle, n = 7 for vehicle+NAC, n = 9 for GGsTop, n = 9 for GGsTop+NAC

L-carnitine synthesis starts with the precursor trimethyl-lysine (TML), which is released from lysosomal protein degradation and ends with hydroxylation of gamma-butyrobetaine (BB) by gamma-butyrobetaine dioxygenase (BBD) producing L-carnitine (Figure 1)

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