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Temperature sensitivity follows standard peptide patterns but with copper-specific considerations

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A comprehensive review published by Lubert Stryer in 1978 entitled Fluorescence energy transfer as a spectroscopic ruler provided a description of the Frsters theory and of the experimental tests of its validity, systemized experimental strategy for the measurements of transfer efficiency, and summarized data on using energy transfer for elucidation of the proximity relationships in a wide variety of biological macromolecules and assemblies, and thereby represented FRET as a powerful tool in the analysis of protein structure and interactions (Stryer, 1978)

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