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The associated expenditures have been substantial: in 2022, the total annual cost of diabetes was $412.9 billion, and obesity-related medical costs in the US were estimated to be nearly $173 billion in 2021

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After pre-conditioning in the presence or absence of 50 nM Exendin-4 for additional 18 hours, culture medium was changed to serum-free DMEM containing 1 mM sodium pyruvate, without glucose and sodium bicarbonate