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The active metabolite, 1,25-dihydroxyvitamin D3 (calcitriol), is generated via sequential hydroxylations in the liver (by CYP2R1) and kidneys (by CYP27B1) (80)

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Utilizing physiologically relevant O 2 -, this approach mimics oxidative stress conditions with high sensitivity ( 3.2.7 Folin-ciocalteu This assay demonstrates operational simplicity, high reproducibility, and sensitivity, making it broadly applicable for estimating the reducing capacity of plant and food extracts