After washing with TBST (50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween-20 and pH 7.6), the fixed cells were blocked using Blocking One reagent (Nacalai Tesque) containing 0.1% Triton X-100 for 1 h at 25 C
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And it raised a concern we kept hearing: if someone is already frail, wont a medication that causes significant weight loss, particularly muscle loss, make things worse
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