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The BPC 157-enhanced cell migration was completely suppressed in the presence of 50 nM hemoglobin, suggesting that the amount of nitric oxide produced by 1.0 g/ml BPC 157 did not exceed 200 nM because hemoglobin contains four heme groups in each molecule

Because of the simplicity of traditional cell culture plates or transwell filters, they are useful for screening multiple compounds and looking at many mechanistic outputs, while the other models are often used for more specialized readouts