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After completion of this 2-level RUVIIIbased normalization, the initially imputed values were removed, then reimputed using the now-normalized data
68 However, dual observations that both inhibition and overactivation of Notch can impede cardiac regeneration underscore the intricacies of Notch signaling in this process
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In a separate set of experiments, heart tissue from NG2-dsRed mice was processed as described above and stained with anti-GLP-1R antibody (Abcam, ab218532, 1:200) following heat-induced epitope retrieval as above
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