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For the Triacsin C treated group, 1 M of the inhibitor was added together with azide-PA and cultured for 24 h

Approximately 90% of GLP-1 is metabolized by DPP-4 before reaching the central venous circulation (Holst and Deacon, 2005), and the peak circulating concentration of active GLP-1 (7-36)NH 2 after a meal in healthy subjects is usually less than 10 pmol/L due to the rapid degradation of DPP-4 ( In order to solve the problem of rapid inactivation of GLP-1 by DPP-4 degradation, GLP-1RAs and DPP-4 inhibitors have been developed to treat T2DM (Kim and Jang, 2015

doi: 10.23736/S0391-1977.16.02565-7

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