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Cells were imaged by time-lapse microscopy using a Zeiss LSM-780 inverted confocal laser-scanning microscope with a 63/1.4 NA oil immersion objective and a 488 nm excitation wavelength and emissions at 508 and 583 nm for 2 minutes to record a baseline, followed by agonist stimulation at 100 nM for 5 minutes and 2-minute exposure to 10 M FSK + 100 M IBMX to generate a maximal response

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Plan on being at our facility for about 1 12 hours from arrival to departure (although this may vary depending on the day/procedure/location ex

Differences between liraglutide and dulaglutide were tested using Student's t -test or Wilcoxon rank-sum test