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doi: 10.1371/journal.pone.0195687 2 ChienYLHwuHGHwangTJHsiehMHLiuCCLin-ShiauSYet al
Cells were subsequently mounted with PermaFluor and visualized using an inverted fluorescence microscope (Nikon Eclipse Ti) at 20 or 40 magnification
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Additionally, H&E and Safranin-O/Fast green staining showed that metformin treatment did not significantly ameliorate cartilage degradation induced by MIA-induced OA in Nrf2 KO mice (Fig
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