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After anesthesia with sodium pentobarbital (50 mg/kg) and analgesia with 1 mg/kg sustained-release buprenorphine (subcutaneous injection, every three days) and 5 mg/kg carprofen (subcutaneous injection, daily), mice were placed on a brain stereotaxis instrument, then a 5 mm midline incision was made over the skull, and a craniotomy was made on the central aspect of the right parietal bone ( in vivo experiments and tissue samples collection were performed by double-blind experiment

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