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Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase(HRP) conjugate are added successively to each micro plate well and incubated

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All experimental procedures were performed in accordance with the European Union Guidelines laid down in the 1964 Declaration of Helsinki and were approved by the Institutional Review Board of the University of Thessaly (Larissa, Greece)

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