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Nrf2 controls the expression of several enzymatic antioxidants and results in the modulation of the levels of ROS, such as SOD, CAT, GPx, and heme oxygenase-1 (HO-1), which are vital in maintaining redox balance and cellular homeostasis

Following incubation at 60C overnight in 10 mM sodium citrate buffer (pH 6.0) for antigen unmasking, BLOXALL blocking solution was added to inactivate endogenous peroxidase (10 min at room temperature (RT))

Diagnostic Tools There are specific questionnaires and scales designed to assess sexual desire and diagnose HSDD, such as the Female Sexual Function Index (FSFI)