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Psoriasis and comorbidities: links and risks

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To confirm whether recombinant proteins of AsGGT1, AsGGT2, and AsGGT3 were expressed as mature GGT enzymes in yeast cells, we first examined deglutamylation activities of these recombinant proteins by using the standard procedure that utilizes -glutamyl- p -nitroanilide, a common synthetic -glutamyl donor substrate for known GGTs (Orlowski and Meister, 1963)