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They're needed to protect cells against oxidative stress and free radicals generated as a natural byproduct of metabolism, environmental exposures, and everything else under the sun

Colony-formation assay One thousand cells were seeded in a 60-mm plate and cultured DMEM supplemented with 10% bovine calf serum for 2 weeks

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Funding This research did not receive any specific grant from funding agencies in public, commercial, or not-for profit sectors