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Moreover, ELISA assays of cytokines in the supernatant of splenic lymphocyte cultures showed that the group immunized with LL-plSAM-FVpE or SAM-FVpE plus PA had significantly elevated IFN- ( Figure 6D ), IL-4 ( Figure 6E ) and IL-17 ( Figure 6F ) levels, compared with the group vaccinated with LL-plSAM or the SAM plus PA