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The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)

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49:08 The Values Behind Their Success 51:18 Future Guests & Final Thoughts We cover: How it all started JD's wife relapsed six months after they got married a rough patch that changed everything A phone call from a man in AA stopped JD from making a decision he would have regretted forever To support his wife, JD started attending a Monday night AA meeting and that's where he met Will's sponsor Will had just gotten out of jail after a fentanyl overdose got a sponsor, started going to meetings Will's sponsor brought him to JD's house for a Wednesday night book study at 6PM Two guys became three, then five, then COVID hit and meetings shut down JD's living room turned into a 70+ person AA meeting every Wednesday night Without that relapse, without that meeting none of this exists How fitness connected them Both were deep into health, supplements, and fitness After meetings they'd talk for hours about compounds, diets, what was working Will was always running something JD was always curious about it That passion for health was the bridge from friendship to business How the podcast happened JD started a fitness channel and wanted to interview Will in his garage first video was on HGH and MK-677 Second video in the backyard