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For ultravioletvisible measurements, one-step in vitro reactions with the standard reaction conditions as defined above were performed in a total volume of 1.2 ml in 2 ml microcentrifuge tubes incubated at 30 C for 15 min at 1,200 rpm using a 3 mm shaking diameter

Tail vein samples were obtained for immediate glucose measurement as above, or into lithium heparin-coated microvette tubes for plasma insulin measurement using a mouse insulin-specific HTRF (Cisbio)

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Micronutrients Assessment, requirements, deficiencies, and interventions