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In animal models (rats), its effects on the nitric oxide (NO) pathway through modulation of NO synthase (eNOS) expression have been described

[DOI] [PMC free article] [PubMed] [Google Scholar] 45.Bao Y.W., Yuan Y., Chen J.H., Lin W.Q

September 24, 2021

Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95