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PT-141 is a synthetic peptide derived from Melanotan II and acts as a melanocortin receptor agonist, particularly at MC3R and MC4R

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Gene expression levels were quantified by qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Chatsworth, CA, USA) and reactions were performed on a Viia TM 7 real time PCR system (Life Technologies, Gaithersburg, MD, USA)

Brown adipose tissue (BAT) is a specialized fat depot characterized by increased energy expenditure and heat production (95)