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This further reveals that increased taurine in the gut is beneficial to ameliorate aging-related dysfunctions like adiposity and leaky gut, which in turn improves healthy life-span

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Briefly, 0.1 ml of supernatants (2.5 mg protein/ml) mixed with 0.1 ml of 15 mM DTT in 100 mM Tris/HCl (pH 9.0), was placed in a 15 ml centrifugation tube, then sealed and incubated at 37 C for 50 min

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