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The copper ion coordinates with nitrogen from the imidazole side chain of histidine, the alpha-amino group of glycine, and the deprotonated amide nitrogen of the glycine-histidine peptide bond, forming a square-planar pyramid configuration

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On a U-100 insulin syringe, 1 mL = 100 units, so 2 mL fills the syringe to 200 units

Animal care was in compliance with standard operating procedures (SOPs) of the Pharmacology animal facility, and the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (ETS 123)