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The GLP-1 peptide found in all other mammalian species binds the human GLP-1R with an IC50 of 7.9 10 10 M (Table 2), whereas the monotreme GLP-1 peptides bind the human and platypus GLP-1R with 50 fold lower affinity

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Preclinical models show dose-dependent synaptic changes (268)

This evolutionary path is fundamentally different from the generally accepted idea that conflicting functions in a single gene favour duplication and diversification, as is the case for Exendin-4 in gila monster