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After 24Lh incubation at 37LC, valproic acid sodium salt (Sigma-Aldrich, P4543) was added to the culture at a final concentration of 2.2LmM, and cells were allowed to grow at 37LC for an additional 24Lh before collection

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Pulmonary fibroblasts were isolated from the 24-week-old fetus, and it was observed that these fibroblasts appeared to lose their proliferative function at the 34th passage

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