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Identified peptides (at least two valid values in technical triplicates) were mapped to GCG, and the confidence of identification is provided via the Andromeda score [29]

doi: 10.1002/dmrr.3070

Until the recent breakthrough on preparation of 15 N-labeled cobalamins via biosynthesis [8] and further development of the first multiple stable isotope dilution assay (SIDA) that simultaneously determines the four natural vitamers [7], the quantification of B12 in foods has been relying on methods mainly applying microbiological assays [9, 10], (U)HPLC-UV [11,12,13,14,15,16,17], and occasionally LC-ICP-MS [18]

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