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Review Oxidative stress and PCOS Oxidative stress is defined as an imbalance in the level of oxidants and antioxidants in the body and is measured with the help of the following parameters: total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) [10]

Misregulation of this process leads to iron overload, which catalyzes the Fenton reaction and exacerbates lipid peroxidation

Quality control of inductively coupled plasmatriple quadrupole mass spectrometer measurements was ensured through a combination of repeat measurements of: Acid blanks A quality control standard made from 1,000 mg l 1 High-Purity Standards multi-element standard solutions A tenfold dilution of the CRM-TMDW (trace metal drinking water) from High-Purity Standards Analyte measurements were normalized to the internal standard Iridium ( 193 Ir) to account for instrument drift and matrix effects, and measurements were subsequently blank corrected by removing the average analyte intensity of repeat blank measurements

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