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During the equilibration phase step, the temperature was controlled by the V-rescale thermostat algorithm 36 with a time constant of 1.0 ps, and the pressure was controlled by the Berendsen coupling algorithm 37 with a time constant of 5.0 ps

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3 Model based estimates based on a mixed model for repeated measures including treatment (and stratification factors for Study 3 only) as a factor and baseline values as a covariate

We first studied hyperpolarized [1- 13 C]dehydroascorbate in the normal rat brain, demonstrating its robust conversion to [1- 13 C]vitamin C, consistent with rapid transport of the oxidized form across the blood-brain barrier