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There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes

At Week 8, patients in the combination treatment group showed significantly greater improvement in total number of lesions compared to the other study groups, suggesting that antibiotic treatment with adjuvant probiotics may offer synergistic anti-inflammatory effects and reduce potential gastrointestinal adverse effects of prolonged antibiotic therapy