Before TBI, male animals drank water with ampicillin (1 g/L), vancomycin (500 mg/L), ciprofloxacin (200 mg/L), imipenem plus cilastatin (250 mg/L), and metronidazole (1 g/L) for 2 weeks, and then the antibiotic treatment was discontinued and replaced by sterile tap water 72 h before the injury
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