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In all cases, the selected and analysed areas were normalised to the number of total pixels selected and the blue channel was used as the threshold to remove the background from hematoxylin staining and orange and red were used to calculate positive pixel density based on the following formula: Iron measurement of human brain tissue Brain tissue was obtained from the NeuroBiobank of the Institute Born-Bunge (NBB-IBB), Wilrijk (Antwerp), Belgium (ID: BB190113), and donors gave informed consent to donate their brains to the NBB-IBB

Monitoring includes regular glucose testing, IGF-1 levels, metabolic panels, insulin sensitivity markers, and clinical assessments