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The protein family is evolutionarily conserved in nearly all eukaryotes, only missing in a few, highly diverged eukaryotes such as Giardia lamblia and Encephalitozoon cuniculi 4

ERK phosphorylation assay HuCCR8.Jurkat cells were incubated in culture media with indicated concentrations of anti-CCR8 antibody for 30 min at 37 C and then 20 nM CCL1 was added for 5 min

doi: 10.1002/bies.201700012 74