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Description

Hormone Metabolic Res

The two elution fractions were mixed and dialyzed overnight in dialysis buffer 1 (20 mM TrisHCl (pH 8.0), 300 mM NaCl, 10% glycerol and 1 mM DTT) and 8 h in dialysis buffer 2 (20 mM TrisHCl (pH 8.0), 150 mM NaCl, 10% glycerol and 1 mM DTT)

Then, in December 2017, the U.S

Off-target detection To determine the site-specific cleavage of the CRISPRCas9 system in vivo, the potential off-target loci were searched using Cas-OFFinder ( The selected potential off-target sites were initially PCR-amplified and then subjected to a DNA sequencing assay