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The sample from each treatment condition was distributed equally into twelve tubes and subjected to heating at different temperatures (the first 9 tubes with one gradient cycle with temperatures 37.0, 41.7, 45.8, 49.7, 53.5, 57.4, 61.3, 64.3, 67.0 C and the remaining 3 tubes with a second gradient cycle with temperatures 70.0, 73.6, 82.7 C to completely precipitate PDE) in the MasterCycler EpGradient S (Eppendorf SE, DE)

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