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After three washes with PBS, the sections were pre-incubated in blocking solution (5% serum albumin, 2% normal goat serum, and 0.3% Triton X-100 in PBS) for 20 min at room temperature, then incubated with an anti-c-Fos rabbit antibody (#sc-52, 1:2000

Additionally, there was variation in the quality of included studies as the risk of bias, methodological rigor, and data integrity were not well tracked or standardized across the available articles

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