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0.3 million cells per sample were incubated with the complex in wash buffer (PBS with 2% BSA) for 30 min at 4 C followed by incubating with the detection antibody (Supplementary Table 2) for another 30 min at 4 C in the dark after washing the excess amount of the complex

Signs include stomach growling or low energy

Understanding the factors that influence the length of treatment, the potential benefits versus risks of long-term use, and the considerations involved in tapering off GLP-1 medication can provide valuable insights for individuals managing their weight loss journey