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In whole-SPZ ELISA, 10 4 SPZs/well or salivary glands insoluble fraction (equivalent to those that yielded 10 4 SPZs) from uninfected mosquitoes were fixed to the wells of microtiter plates and overlayed with either 50% HIS (heat inactivated serum) in VBS ++ (1x veronal buffered saline, pH 7.3, 0.15mM CaCl 2 , 0.5mM MgCl 2 ) or VBS ++ alone

Plasma concentrations return to baseline within 12 hours after intravenous administration of 0.5 g [5][12]