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cAMP accumulation assay Modulation of the intracellular second messenger cAMP was analysed by use of Adenylyl Cyclase Activation Flashplate Assay in whole cells stably over-expressing the respective receptor (BHK-hGLP-1R, BHK-hGCGR, BHK-hGIPR, BHK-hGLP-2R) according to manufacturers instructions (Perkin Elmer)

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For the next decade, growth was deliberate