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Reconstitute using sterile bacteriostatic water or an appropriate laboratory buffer Add solvent slowly along the vial wall to minimize foaming during dissolution Avoid vigorous agitation, shaking, or vortexing of the peptide solution Gently swirl or invert the vial until the peptide is fully dissolved Store reconstituted solutions at 3646 F (28 C) during short term use Prepare aliquots where appropriate to reduce repeated freezethaw exposure Careful reconstitution practices help maintain peptide stability and support reliable experimental conditions

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Real-time PCR was carried out using SYBR Green PCR Master Mix (RR091A, Takara, Japan) and the quantitative analysis was performed using LightCycler Roche 480 qPCR instrument