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Surgery Mice, under isoflurane anaesthesia and receiving Metacam before surgery, were stereotactically implanted with a temporary guide cannula (Plastics One) positioned above the AP (anterior/posterior (A/P), 0.5 mm

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While high-intensity focused ultrasound is associated with low recurrence rates, CA may be preferable due to its real-time monitoring of ice build-up, which minimizes collagen matrix damage and its associated tissue granulationultimately leading to enhanced long-term esthetic results [20]