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Cell culture Bone marrow-derived macrophages (BMDMs) were harvested from the femur and tibia of C57BL/6J mice and cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 10% L929 cell-conditioned medium, and maintained in a humidified incubator with 5% CO 2 at 37 C for 7 days [36]

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Furthermore, experimental evidence has shown that GDF15 may influence pain modulation by reducing nociceptive neuron excitability through peripheral mechanisms involving Nav1.8 sodium channel suppression and modulation of protein kinase A (PKA) and extracellular signal-regulated kinase (ERK) signaling pathways 26,27,28,29

Even if supplements are considered natural, that doesnt necessarily mean theyre safe, concludes Dr