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Statistical significance was determined by two-way ANOVA followed by Bonferroni post-hoc analysis

In this context, although while studies conducted in meta-analyses suggest that a consistent circadian rhythm is not observed, possibly due to small sample sizes, a recent study reported that sphingolipids and phospholipids exhibit higher concentrations in the evenings, with diacylglycerols and triacylglycerols showing more pronounced circadian variation compared to phospholipids and sphingolipids [3, 33]