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Results HBV genotyping and methylation profile analysis of the HBV DNA derived from different HCC cell lines by BS-PCR sequencing To obtain the comprehensive methylation profiles for each CpG site of all three known CpG islands in the HBV genomes from both cell cultures and infected tissues, we chose to perform bisulfite PCR (BS-PCR) sequencing on a small set of tissue samples (8 hepatitis, 6 cirrhosis and 12 HCC) based on the quantity of DNA available to us