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Notably, the 1,4-dihydropyridine ring oxidized analogue (KL7) completely lost the adjuvant activity, presumably due to altered electronic structure, reduced conformational flexibility, or lost hydrogen bonding capacity (Extended Data Fig

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Determination of lactate dehydrogenase (LDH) activity bFLS were treated with bTNF- or vehicle, and after 6 h, were homogenized on ice in 500 l of cold lactate dehydrogenase (LDH) assay buffer