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The reverse transcription conditions were 42 C for 3050 min, followed by 85 C for 5 s

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Whole aorta en face imaging showed that neither vehicle-treated nor semaglutide-treated groups exhibited reduced aorta plaque area compared with the baseline controls (Supplemental Figure 2B), even though semaglutide administration caused body weight loss but did not reduce liver or spleen weight (Supplemental Figure 2, C and D)

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