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Transfection efficiency was detected by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blot 48 h after transfection and compared with cells transfected with the corresponding negative control vector or negative control siRNA

Intensive insulin therapy prevents the progression of diabetic microvascular complications in Japanese patients with non-insulin-dependent diabetes mellitus: a randomized prospective 6-year study

ACE (Angiotensin I converting enzyme), which converts angiotensin I to angiotensin II, is involved in blood pressure regulation 53 and has shown roles in intestinal homeostasis, including terminal digestion of peptides 49 , cell turnover 54 and glucose uptake inhibition via local production of angiotensin II 55

Shibayama, K