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This ultimately results in balancing not only estrogen but other important hormones such as progesterone

The dephospho-CoA kinase (DPCK) domain of COASY catalyzes the final step in CoA synthesis by adding the phosphate from ATP to the 2-hydroxyl of the ribose from the adenine added in the previous reaction

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After the reaction was complete, the solvent was distilled off under reduced pressure, and the residue was redissolved with ethyl acetate, extracted with 1M hydrochloric acid solution (50mL2), saturated sodium bicarbonate solution (50mL2), washed with saturated brine (75mL), The organic layer was collected and dried over anhydrous sodium sulfate