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Statistical analysis Demographic and clinical characteristics across groups were assessed using independent-samples t-tests, analysis of variance (ANOVA), and chi-square tests, or Fishers exact test, as appropriate

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Suspensions were centrifuged at 5,000 g for 5 min at 4C, supernatants were transferred to a new tube, and TCA buffer was added to a final concentration of 33% and incubated at 4C overnight to precipitate histone proteins