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The tube was centrifuged for 8 min at 3,400 rpm (again) and decanted into a 200 ml round bottom flask and 2 ml of toluene added for vapor exchange
The results imply that even residues not directly involved in the interaction may exhibit changes in their chemical environment if they are adjacent to residues crucial for the interaction or undergo conformational changes upon binding 39,40
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Prevention of glomerular dysfunction in diabetic rats by treatment with d-alpha-tocopherol
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