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The library pool was quantified by real-time PCR using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System and then sequenced on an Illumina NovaSeq6000 sequencing instrument with a paired-end 2 150 bp

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Moreover, from the distribution curve it can be seen that except for FAD all the three phytochemicals showed a unimodal distribution with a peak value of 232 nm 2 , 241 nm 2 and 243 nm 2 for Sesamin, Quercitrin and Quercetin respectively (Fig