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These alterations in the tumor ECM induce an increase in its stiffness, typically evaluated using techniques that either require direct contact with the sample (e.g., atomic force microscopy), or measure the tumor mechanical properties in a contact-free manner (e.g., shear wave elastography, magnetic resonance elastography) (Table 3)

Smoking reduces the number of sperm, their ability to move, and their likelihood of having normal shape and structure

Maintaining adequate nutrition is paramountdespite reduced appetite, it is essential to consume sufficient calories and nutrients to support basic metabolic functions

This minimizes the risk of introducing contaminants